Table 1 Distribution of mutant and wild type alleles in the samples sequenced by Sanger and NGS methods
Gene | Mutations | Sanger number (%) | NGS number (%) | ||
|---|---|---|---|---|---|
Mutant allele | Wild type | Mutant allele | Wild type | ||
Pfcrt | M74I | 7 (26.9%) | 19 (73.1%) | 9 (34.6%) | 17 (65.4%) |
N75E | 7 (26.9%) | 19 (73.1%) | 9 (34.6%) | 17 (65.4%) | |
K76T | 7 (26.9%) | 19 (73.1%) | 9 (34.6%) | 17 (65.4%) | |
A220S | NA | NA | 7 (26.9%) | 19 (73.1%) | |
Q271E | NA | NA | 6 (23%) | 20 (77%) | |
I356T | NA | NA | 8 (30.8%) | 18 (69.2%) | |
R371I | NA | NA | 7 (26.9%) | 19 (73.1%) | |
Pfmdr1 | N86Y | 2 (7.7%) | 24 (92.3%) | 2 (7.7%) | 24 (92.3%) |
Y184F | 17 (65.4%) | 9 (34.6%) | 22 (84.6%) | 4 (15.4%) | |
N504K | NA | NA | 4 (15.4%) | 22 (84.6%) | |
N649D | NA | NA | 3 (11.5%) | 23 (88.5%) | |
F938Y | NA | NA | 3 (11.5%) | 23 (88.5%) | |
S967N | NA | NA | 1 (3.8%) | 25 (96.2%) | |
Pfk13 | Q613H | Nil | 100% | 1 (3.8%) | 25 (96.2%) |
K189T | NA | NA | 17 (65.4%) | 9 (34.6%) | |
H136N | NA | NA | 1 (3.8%) | 25 (96.2%) | |