- Media composition of RPMI-1640, Waymouth and McCoy5A from commonly available commercial brands. Shadowed are components of special interest. Waymouth medium is available either powder or liquid and it is highly enriched in amino-acids, ascorbic acid and glutathione. With a higher concentration in glucose than most other commercial media. It is also the only medium used for cultivating P. vivax that includes hypoxanthine in its formulation. RPMI-1640 can be sourced in either its powder or liquid forms from a number of different companies. Modified versions of this medium are also commercially available: in such versions the RPMI-1640 is typically supplemented with or depleted of l-glutamine, sodium bicarbonate, HEPES (15 mM, 20 mM or 25 mM), folic acid, methionine, cysteine, Glutamax as well as different amounts of glucose. Special formulations known as the ATCC modification (containing high glucose, low sodium bicarbonate, sodium pyruvate, HEPES, and low l-glutamine) or the Dutch modification (addition of HEPES, with lower sodium bicarbonate (1 g/L)) are also obtainable. McCoy’s 5A, unlike other media, contains bacto-peptone, and high levels of glucose. Moreover, it contains high levels of folic acid and thiamine while the levels of cysteine and leucine are reduced compared to other media formulations. McCoy5A contains no magnesium chloride and lower levels of glutathione compared to RPMI and Waymouth. Last, McCoy5A comprises an increased concentration of pABA, and folic acid compared to different commercially available RPMI and Waymouth media. Commercially available in powder and liquid forms too, it includes variant formulations comprising the addition of HEPES, Glutamax, l-glutamine and extra sodium bicarbonate
