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Table 1 Results of image analysis, and estimation of antigen concentrations and type of malaria infection from Plasmodium falciparum positive clinical samples

From: A two-colour multiplexed lateral flow immunoassay system to differentially detect human malaria species on a single test line

Sample no. 25 460 463 468 770
Microscopy examination Positive P. falciparum Positive P. falciparum Positive P. falciparum Positive P. falciparum Positive P. falciparum
Red decay area at test line 917.11 1206.18 1335.83 1183.03 799.466
Blue decay area at test line 499.20 627.21 663.44 614.65 448.40
Red/blue ratio of clinical samples (ratio_cal) 1.84 1.92 2.01 1.92 1.78
Estimated red/blue ratio at the red decay area (ratio_ref) 1.95 2.13 2.14 2.12 1.83
LFA colour discrimination Positive P. falciparum Positive P. falciparum Positive P. falciparum Positive P. falciparum Positive P. falciparum
ELISA results (pLDH: μg mL−1; PfHRP2: ng mL−1) pLDH: 56.10
PfHRP2: 66.38
pLDH: *
PfHRP2: > 312.5
pLDH: 20.12
PfHRP2: > 312.5
pLDH: 22.93
PfHRP2: > 312.5
pLDH: 67.74
PfHRP2: 240.65
  1. * > 5.00 μg mL−1 but < 20.00 μg mL−1