Table 1 Results of image analysis, and estimation of antigen concentrations and type of malaria infection from Plasmodium falciparum positive clinical samples

Microscopy examination

Positive P. falciparum

Positive P. falciparum

Positive P. falciparum

Positive P. falciparum

Positive P. falciparum

Red decay area at test line

917.11

1206.18

1335.83

1183.03

799.466

Blue decay area at test line

499.20

627.21

663.44

614.65

448.40

Red/blue ratio of clinical samples (ratio_cal)

1.84

1.92

2.01

1.92

1.78

Estimated red/blue ratio at the red decay area (ratio_ref)

1.95

2.13

2.14

2.12

1.83

LFA colour discrimination

Positive P. falciparum

Positive P. falciparum

Positive P. falciparum

Positive P. falciparum

Positive P. falciparum

ELISA results (pLDH: μg mL⁻¹; PfHRP2: ng mL⁻¹)

pLDH: 56.10

PfHRP2: 66.38

pLDH: *

PfHRP2: > 312.5

pLDH: 20.12

PfHRP2: > 312.5

pLDH: 22.93

PfHRP2: > 312.5

pLDH: 67.74

PfHRP2: 240.65