Fig. 2
From: Ultrasensitive loop mediated isothermal amplification (US-LAMP) to detect malaria for elimination
Validation of Pan US-LAMP assay on serially diluted clinical specimens. US-LAMP assay was carried on whole blood (a, c, e) and dried blood spot (DBS) extracted total nucleic acid (b, d, f). Here, a–f summarizes the data obtained from P. falciparum, P. vivax and P. ovale spp. serial dilutions prepared from clinical specimens. Data was obtained from triplicate experiments where error bars indicate standard error of mean (SEM). Threshold time (min = minutes) was determined by placing the threshold bar at 200 RFU in the CFX96 Real Time system. R2 indicates goodness of fit to the straight line