Fig. 5
CQGREEN uptake in CQS and CQR parasites. Trophozoite stage parasites were incubated with CQGREEN and fluorescence was recorded every 3 s. a Fluorescence was measured in the parasite cytosol, DV, infected RBC (iRBC) cytoplasm and uninfected RBC (uRBC). No accumulation of CQGREEN was observed for the uRBC and iRBC cytoplasm. Uptake rates were calculated for the parasite cytosol and DV during the saturation phase (black curve), which was followed by a linear uptake phase. b CQR strains showed a slower CQGREEN uptake rate compared to CQS strains in both the cytosol and the DV of the parasite. Addition of verapamil (VP) did not influence the CQGREEN uptake rate in CQR strains. Experiments were done in triplicate