Fig. 2

Characterization of virus-like particles (VLPs). Diagram for influenza VLPs displaying P. berghei AMA1 (a). Electron microscopy of VLPs containing AMA-1 from non-codon optimized (b) and codon-optimized VLPs (c). Western blot analysis for non-codon optimized and codon-optimized VLPs (d, e). VLPs (100 μg, 50 μg, 25 μg) were loaded for SDS-PAGE. Polyclonal mouse anti-P. berghei antibody was used to probe P. berghei AMA-1 protein (1671 bp/46 kDa) and anti-M1 monoclonal antibody was used to determine influenza M1 protein (1027 bp/28 kDa)