Fig. 8

Microscopic observations of H&E staining on day 5 post P. berghei infection in the liver of an uninfected control mouse and P. berghei infected mice following the modulation of IL-35. Uninfected mouse liver showing hepatic artery (HA), portal vein (PV) and normal red blood cells (NRBC). The portal vein in sections from infected mice was congested with PRBC’s, macrophages and lymphocytes (1) in addition to rosettes of PRBC’s (2). Hyperplasia and hypertrophy of stellate macrophages/von kupffer cells (3), haemozoin pigment deposition in liver parenchyma (4), sinusoidal dilatation and congestion with malarial pigment (haemozoin) and PRBC’s (5), hydropic degeneration (6), PRBC sequestration and adherence to endothelial wall (7) were also apparent. Images were acquired at ×400 total magnification (scale bar; 50 µm). Photomicrographs are representative images from three experiments