Quantification of malaria antigens PfHRP2 and pLDH by quantitative suspension array technology in whole blood, dried blood spot and plasma

Table 1 Clinical samples tested on the qSAT assay

Samples (n)	Type of sample	Origin	Sampling period	Population	Reference assay	P. falciparum positive (n)	GM parasite density (p/µl) (95% CI)	Others
765	Plasma	Mozambique	2010–2012	Pregnant women	qPCR on DBS	59	127 (58.4–276.3)
103	Serum	Colombia	2005–2007	Pregnant women	Microscopy	32	3901 (2059–7391)
110	EDTA whole blood	Senegal	NA	Adults	Nested PCR and qPCR on WB^a	55	4.9 (2.6–9.4)
16	EDTA whole blood	Peru	April–Aug 2015	Adults	Nested PCR and qPCR on WB^a	16	52.7 (19.1–146.0)	pfhrp2 deleted
639	EDTA whole blood	Nigeria	April–Aug 2017	Age ≥ 5 years	Microscopy and PCR^b	639	4713 (3530–6292)
323	EDTA whole blood	Peru	March–Oct 2017	Age ≥ 5 years	Microscopy and PCR^b	323	1719 (1328–2225)
75	Plasma	Spain	2010	Pregnant women	NA	NA	NA

GM Geometric mean
^aNested PCR was used for species determination and qPCR for parasite density quantification. Plasmodium falciparum samples were positive for both methods
^bPCR was used for species determination and microscopy for parasite density quantification. Geometric mean parasite density is calculated on the basis of microscopy positive samples (n = 247/639 in Nigeria, and 191/323 in Peru)

ISSN: 1475-2875