Fig. 1

Close proximity response assay. a Phylogenetic relationship of the three mosquito species used in this study (adapted from Sieglaff et al. [17]). b A schematic of the close proximity response assay. An individual mosquito is introduced into the cage. The mosquito lands on the mesh wall of the cage and is allowed to rest for at least 5 min for acclimatization, before starting the experiment. A pipette tip containing a piece of filter paper soaked with the odorant is placed on the opposite side of the mesh wall (filter paper is 0.5 cm away from the mosquito), and the mosquito is monitored for 30 s