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Fig. 7 | Malaria Journal

Fig. 7

From: Comparison of ELISA with electro-chemiluminescence technology for the qualitative and quantitative assessment of serological responses to vaccination

Fig. 7

Linearity of ELISA vs ECLIA based assay for the assessment of CSP-specific antibodies. a ELISA readout for single sample (human CSP-immune pool) across fifteen twofold dilutions from 1:50 to 1:819,200, compared against the relative serum concentration, expressed as the log Dilution−1 for the CSP-FL, CSP C-term, and NANP antigens. Dilution points that were found to be in the linear range are marked (solid circles), with corresponding R2 values. b ΔOD/ΔC values calculated between adjacent dilutions against relative serum concentration for the ELISA. ΔOD/ΔC curve estimates are shown as well. c ECLIA assay readout for a single sample (human CSP-immune serum pool) across eight fivefold dilutions against CSP-FL, CSP C-term, and NANP against the relative serum concentration, expressed as the log Dilution−1. Five dilution points (solid circles) were found to be in the linear range for each antigen, with corresponding R2 values shown. d ΔI/ΔC values calculated between adjacent dilutions against relative serum concentration for the ECLIA. ΔI/ΔC curve estimates are shown as well

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Malaria Journal

ISSN: 1475-2875

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