Table 1 Summary of features characteristic to ELISA vs. ECLIA
Parameters | ELISA | ECLIA |
|---|---|---|
Cost of hardware | Starting at $13,000 | Starting at $54,000 |
Cost of assays | High (due to singleplexing) | Medium (high throughput and multiplexing) |
Throughput | 24 dilutions of samples per plate (Triplicates) → 6 samples/plate | 80 samples/plate (singlicates); |
8 plates/day → 48 samples/day | 8 plates/day → 640 samples | |
Operator skill | Basic | Basic |
Training time | Weeks | Weeks |
Hands-on time | 1 h | 2 h |
Asssay length | 5 h (plus ON plate coating) | 6.5 h |
Reporter | Enzymatic | Electro-chemiluminescence |
Detection | Colorimetry | Luminescence |
Results reported | OD1 titer | Mean Luminescence Signal |
Endpoint titer | Titer | |
Concentration | Concentration | |
Chemistry | Absorption to protein binding plates | Biotinylated antigen binding to U-PLEX linkers |
Assay format | Plate based | Plate based |
Multiplexing | No | Yes |
Number of analytes | 1 analyte/well | 1–10 analytes/well |
Serial dilutions needed | Yes | No |
Sample volume | > 5 µl sample for one antigen (in most instances) | < 0.1 µl sample for 10 antigens |
Linear range | Narrow | Wide |
Sensitivity | Low | High |