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Table 1 Summary of features characteristic to ELISA vs. ECLIA

From: Comparison of ELISA with electro-chemiluminescence technology for the qualitative and quantitative assessment of serological responses to vaccination

ParametersELISAECLIA
Cost of hardwareStarting at $13,000Starting at $54,000
Cost of assaysHigh (due to singleplexing)Medium (high throughput and multiplexing)
Throughput24 dilutions of samples per plate (Triplicates) → 6 samples/plate80 samples/plate (singlicates);
8 plates/day → 48 samples/day8 plates/day → 640 samples
Operator skillBasicBasic
Training timeWeeksWeeks
Hands-on time1 h2 h
Asssay length5 h (plus ON plate coating)6.5 h
ReporterEnzymaticElectro-chemiluminescence
DetectionColorimetryLuminescence
Results reportedOD1 titerMean Luminescence Signal
Endpoint titerTiter
ConcentrationConcentration
ChemistryAbsorption to protein binding platesBiotinylated antigen binding to U-PLEX linkers
Assay formatPlate basedPlate based
MultiplexingNoYes
Number of analytes1 analyte/well1–10 analytes/well
Serial dilutions neededYesNo
Sample volume> 5 µl sample for one antigen (in most instances)< 0.1 µl sample for 10 antigens
Linear rangeNarrowWide
SensitivityLowHigh