Table 1 List of primer and conditions used for PCR amplification of portions of the voltage gated sodium channel and acetylcholinesterase genes
From: Insecticide resistance in Anopheles stephensi in Somali Region, eastern Ethiopia
Assay | Primer | Sequence | Annealing temperature (°C) | Final concentration (µM) |
|---|---|---|---|---|
Voltage gated sodium channel | ||||
| Â | KdrF | GGACCAYGATTTGCCAAGAT | 50 | 1.25 |
| Â | VGS_1R | CGAAATTGGACAAAAGCAAGG | 50 | 1.25 |
Acetylcholinesterase | ||||
| Â | Ex3AGdir | GATCGTGGACACCGTGTTCG | 56 | 1 |
| Â | Ex3AGrev | AGGATGGCCCGCTGGAACAG | 56 | 1 |