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Table 1 List of primer and conditions used for PCR amplification of portions of the voltage gated sodium channel and acetylcholinesterase genes

From: Insecticide resistance in Anopheles stephensi in Somali Region, eastern Ethiopia

AssayPrimerSequenceAnnealing temperature (°C)Final concentration (µM)
Voltage gated sodium channel
 KdrFGGACCAYGATTTGCCAAGAT501.25
 VGS_1RCGAAATTGGACAAAAGCAAGG501.25
Acetylcholinesterase
 Ex3AGdirGATCGTGGACACCGTGTTCG561
 Ex3AGrevAGGATGGCCCGCTGGAACAG561