Table 1 List of primer and conditions used for PCR amplification of portions of the voltage gated sodium channel and acetylcholinesterase genes
From: Insecticide resistance in Anopheles stephensi in Somali Region, eastern Ethiopia
| Assay | Primer | Sequence | Annealing temperature (°C) | Final concentration (µM) |
|---|---|---|---|---|
| Voltage gated sodium channel | ||||
| KdrF | GGACCAYGATTTGCCAAGAT | 50 | 1.25 | |
| VGS_1R | CGAAATTGGACAAAAGCAAGG | 50 | 1.25 | |
| Acetylcholinesterase | ||||
| Ex3AGdir | GATCGTGGACACCGTGTTCG | 56 | 1 | |
| Ex3AGrev | AGGATGGCCCGCTGGAACAG | 56 | 1 | |