Fig. 3

Evaluation of the immunogenic properties of CL-vac compared with the two-vial formulation. After reconstitution of the vaccines, mice were immunized three times intramuscularly and the induced specific antibody responses were measured 2 weeks after the third immunization by ELISA (anti-HBsAg and anti-R32LR) (a). The cellular responses (b) were evaluated on pooled blood 7 days after the third immunization by intracellular cytokine staining, measuring the percentage of CSP- and HBsAg-specific CD4 + T cells expressing IFNγ and/or IL-2 and/or TNFα. Immunization with the two-vial formulation was used as control. An investigational two-dose version of CL-vac was used in this experiment. One dot represents one mouse or one pool. The black bars are means for the antibody responses and medians for the cellular responses. The statistical analysis demonstrated the non-inferiority of CL-vac compared with the two-vial formulation, for all four measured parameters