Table 1 List of oligonucleotides and synthetic cRNA used in this study

pfs25 (female gametocytes)

Forward: 5′-AAATCCCGTTTCATACGCTTGTAA-3′

Reverse: 5′-CAGTTTTAACAGGATTGCTTGTATCTAATATAC-3′

Probe: 5′-FAM-ACCAAATGAATGTAAGAATGTAACTTGTGGTAACGGT-BHQ1-3′

P. falciparum pfs25 mRNA

[11]

pfMGET (male gametocytes)

Forward: 5′-AAAATTCGGTCCAAATATAAAATCCTG-3′

Reverse: 5′-CTTCATCAATTAAAAATCCCTTTTTTGT-3′

Probe: 5′-FAM-CCTGGTAAAAAACAGCTCCAGCA-BHQ1-3′

P. falciparum PF14_0063 (PF3D7_1469900) mRNA

[5, 6]

EAV (extraction control)

Forward: 5′-GGCGACAGCCTACAAGCTACA-3′

Reverse: 5′-CGGCATCTGCAGTGAGTGA-3′

Probe: 5′-FAM-TTGCGGACCCGCATCTGACCAA-BHQ1-3′

EAV ORF7

[26]

pfs25 cDNA with T7

5′-TAATACGACTCACTATAGGGAACAAATCCCGTTTCATACGCTTGTAAATGTAATCTTGGATATGATATGGTAAATAATGTTTGTATACCAAATGAATGTAAGAATGTAACTTGTGGTAACGGTAAATGTATATTAGATACAAGCAATCCTGTTAAAACTGTTT-3′

This study

pfMGET cDNA with T7

5′-TAATACGACTCACTATAGGGAACAAAATTCGGTCCAAATATAAAATCCTGTTCAGAAGAAAAAAAAAGTATCCTGGTAAAAAACAGCTCCAGCATTAAAAACACAAAAAAGGGATTTTTAATTGATGAAGTTT-3′

This study