Assays for quantification of male and female gametocytes in human blood by qRT-PCR in the absence of pure sex-specific gametocyte standards

Table 5 pfMGET (male gametocyte) qRT-PCR assay for assessing inter-assay variability between runs for 15 clinical study cohorts

Standard (gametocytes/mL)	n	Mean C_q (SD)	Range C_q	%CV C_q
1.73 × 10⁷	28	16.42 (0.26)	15.76–17.57	1.6
1.73 × 10⁶	29	19.68 (0.28)	19.11–20.90	1.4
1.73 × 10⁵	29	22.97 (0.28)	22.32–24.13	1.2
1.73 × 10⁴	29	26.32 (0.33)	25.65–28.07	1.3
1.73 × 10³	29	29.71 (0.34)	28.85–31.30	1.2
1.73 × 10²	29	33.08 (0.64)	32.11–35.42	1.9
1.73 × 10¹	21^a	36.30 (0.90)	34.57–37.67	2.5
Total	194	26.00 (0.46^b)	15.76–37.67

C_q: quantification cycle, SD: standard deviation, %CV: percent coefficient of variation
^aOut of 29 runs
^bOverall SD

ISSN: 1475-2875