Fig. 4

Treatment with CoPPIX decreases lung pathology. Animals received daily i.p. injection of saline with 0.5% DMSO (Saline), or 5 mg/kg CoPPIX (CoPPIX) and were euthanized 9 days after infection. a Total leukocyte counts. b, c represent differential leukocyte counts. Mononuclear cells (b) and polymorphonuclear cells (c). Each bar composed of five animals. d–g. Macroscopic aspect of lungs and photomicrographs from control and infected animals treated or not with CoPPIX. Photos are representative of five animals per experimental group, and three independent experiments. Magnification of ×10. The bar on the lower right corner of the photomicrographs indicates 100 µm. In H, protein content in the BALF. In I, Evans blue extravasation to the lung tissue (with macroscopic photos of the lungs representative of each group). Each bar averaging to 8–10 animals. The experiments were repeated 3 times with similar results. *p ≤ 0.05 when compared to uninfected controls, and (#) indicates p ≤ 0.05 when compared to animals treated with CoPPIX. P values were calculated using an unpaired Ordinary one-way ANOVA test