Genetic polymorphism of Plasmodium falciparum circumsporozoite protein on Bioko Island, Equatorial Guinea and global comparative analysis

Table 2 Global PfCSP C-terminal Mutant types and effect prediction

Mutant	Frequency in global (X/2296)	Frequency in Bioko (X/96)	SIFT	Humdiv	ΔΔG
K314Q	21.78%	47.92%	0.13	0.997A	− 0.373664
K317E	84.32%	93.75%	0.43	0.146C	1.07621
K317T	6.97%	1.04%	0.2	0.995A	1.20284
K317A	0.17%	0	0.08	0.988A	1.31391
E318K	23.52%	56.25%	0.08	0.04C	1.29697
E318Q	59.67%	34.38%	0.06	0.02C	0.981937
N321K	84.76%	79.17%	0.84	0.043C	− 1.74619
N321Q	1.83%	6.25%	0.54	0.954A	− 0.111155
N321T	2.92%	0	0.51	0.082C	− 1.89854
K322E	1.61%	8.33%	0.09	0.186C	0.882041
K322I	5.97%	5.21%	0.02*	0.353C	− 0.667251
K322R	4.44%	10.42%	0.16	0.521B	0.504493
K322T	16.9%	43.75%	0.06	0.186C	1.11661
K322N	6.45%	0	0.06	0.186C	1.04753
Q324K	19.29%	39.58%	0.29	0.043C	− 0.294208
N325Y	7.58%	0	0.02*	0.186C	0.241653
S326A	0.09%	0	0.04*	0.94B	− 0.00308024
L327I	11.06%	10.42%	0.82	0.994A	1.56456
G349D	0.13%	0	0.83	0.999A	− 0.608967
N352G	8.36%	9.38%	0.14	0.994A	0.117207
N352D	18.73%	6.25%	0.08	0.523B	0.0203338
P354S	4.66%	1.04%	0.18	0.988A	1.85106
D356N	3.79%	9.38%	0.1	0.995A	− 0.222874
D356G	0.09%	2.08%	0.09	0.988A	0.0325801
E357Q	45.78%	76.04%	0.13	0.723B	− 0.334979
D359N	8.84%	14.58%	0.2	0.288C	0.433524
A361E	72.43%	46.88%	0.79	0.186C	0.273329
A361I	0.96%	1.04%	0.46	0.973A	0.206936

* SIFT < 0.05, predicted to be deleterious; HumdivA > = 0.953, probably damaging; 0.953 > HumdivB > = 0.432, possibly damaging; 0.432 > HumdivC > = 0.0024, benign

ISSN: 1475-2875