An unlabelled probe-based real time PCR and modified semi-nested PCR as molecular tools for analysis of chloroquine resistant Plasmodium vivax isolates from Afghanistan

Table 2 High-resolution melting assay primer and probe sequences used for detection K10 insertion in pvcrt-o gene

Name	Primer/probe	Sequence 5′→3′	TM	Products size
Name	Primer/probe	Sequence 5′→3′	TM	Wild	Mutant
Pvcrto-O F	Forward primer	GCTACCCCTAACGCACAATG	60	80	83
Afg.HRM. R	Reverse primer	CCGGTAACGTTCATCGG
Afg.U.P	Unlabelled Probe	CTGAAAAAGAAGAAGAAGAAGGG- block

ISSN: 1475-2875