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Table 4 Cycle threshold (Ct) values for the 1st WHO International Standard samples tested using the HIV/HCV/HBV/malaria NAT assay (Bio-Manguinhos)

From: Use of a NAT-based assay to improve the surveillance system and prevent transfusion-transmitted malaria in blood banks

Replicate

Concentration tested

2.00E02 (Ct value)

1.00E02 (Ct value)

5.00E01 (Ct value)

2.50E01 (Ct value)

1

32.56

33.66

33.79

35.75

2

32.63

32.94

36.24

43.99

3

32.39

32.88

37.39

34.23

4

31.92

32.76

34.42

47.63

5

32.39

33.31

34.17

34.96

6

32.05

33.73

34.48

37.67

7

32.73

33.17

33.23

ND

8

33.1

33.31

33.64

35.39

Mean (SD)

32.47 (± 0.38)

33.22 (± 0.35)

34.67 (± 1.42)

38.52 (± 5.2)

  1. ND not detected, SD standard deviation