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Table 1 Mean parasitaemia, developmental stages differential proportions, ring ratios and survival proportion of ten Plasmodium vivax Aotus-derived ex vivo cultures maintained with modified McCoy’s5A supplemented with 25% serum alone or 20% serum in combination with 5% chemically defined lipid concentrate

From: On the survival of 48 h Plasmodium vivax Aotus monkey-derived ex vivo cultures: the role of leucocytes filtration and chemically defined lipid concentrate media supplementation

Time (hours)

Group

Mean parasitaemia

Ring Ratio

Survived/total

%

Rings  %

Trophozoites  %

Schizonts  %

Gametocytes %

0

FS

0.4

54

31

14

1

1.17*

10/10

FL

0.4

52

40

8

0

1.08*

10/10

RS

0.7

39

45

15

1

0.64

10/10

RL

0.7

40

46

13

1

0.67

10/10

24

FS

0.2

49

45

6

0

0.96

10/10

FL

0.2

39

49

11

1

0.64

10/10

RS

0.4

37

42

21

0

0.59

10/10

RL

0.5

40

40

20

0

0.67

10/10

48

FS

0.1

13

41

46

0

0.15

6/10

FL

0.1

28

72

0

0

0.39

5/10

RS

0.1

40

27

33

0

0.67

8/10

RL

0.2

35

35

30

0

0.54

9/10**

  1. *p = 0.0292; one-tailed Chi square test
  2. **p = 0.0255; one-tailed Chi square test
  3. Ring ratio = rings/mature stages (trophozoites, schizonts and gametocytes)
  4. FS filter serum, FL filtered lipid, RS retained serum, RL retained lipid