Table 3 Prevalence of pfcrt, pfmdr1, pfdhfr and pfdhps haplotypes in P. falciparum isolates from day 3 positive and negative patients after artemether-lumefantrine treatment
Gene | Mutants | Haplotype | n (%) | Pa | ||
|---|---|---|---|---|---|---|
Day 3 positive, n = 4 | Day 3 negative, n = 26 | Total, n = 30 | ||||
pfk13 | Wild type | K189 | 0 (0.0) | 10 (38.5) | 10 (33.3) | 0.272 |
Single | T189 | 0 (0.0) | 3 (11.5) | 3 (10.0) | 1.000 | |
NN K189 | 4 (100.0) | 13 (50.0) | 17 (56.7) | 0.113 | ||
pfcrt | Double | T76S220 | 4 (100.0) | 26 (100.0) | 30 (100) | 1.000 |
pfmdr1 | Wild type | Y184G968 | 3 (75.0) | 19 (73.1) | 22 (73.3) | 1.000 |
Single | F184G968 | 1 (25.0) | 6 (23.1) | 7 (23.3) | 1.000 | |
Y184A968 | 0 (0.0) | 1 (3.8) | 1 (3.3) | 1.000 | ||
pfdhps | Single | S436G437K540A581 | 0 (0.0) | 2 (7.7) | 2 (6.7) | 1.000 |
Double | S436G437E540A581 | 0 (0.0) | 3 (11.5) | 3 (10.0) | 1.000 | |
A436G437K540A581 | 0 (0.0) | 1 (3.8) | 1 (3.3) | 1.000 | ||
Triple | S436G437E540G581 | 0 (0.0) | 1 (3.8) | 1 (3.3) | 1.000 | |
A436G437E540A581 | 4 (100.0) | 19 (73.1) | 23 (76.7) | 0.548 | ||
pfdhfr | Double | N51R59N108I164 | 0 (0.0) | 2 (7.7) | 2 (6.7) | 1.000 |
Triple | N51R59N108L164 | 0 (0.0) | 4 (15.4) | 4 (13.3) | 1.000 | |
I51R59N108I164 | 1 (25.0) | 5 (19.2) | 6 (20.0) | 1.000 | ||
Quadruple | I51R59N108L164 | 3 (75.0) | 15 (57.7) | 18 (60.0) | 0.632 | |
pfdhps–pfdhfr | Quadruple | S436G437K540A581–N51R59N108L164 | 0 (0.0) | 2 (7.7) | 2 (6.7) | 1.000 |
S436G437E540A581–N51R59N108I164 | 0 (0.0) | 1 (3.8) | 1 (3.3) | 1.000 | ||
Quintuple | S436G437E540A581–I51R59N108I164 | 0 (0.0) | 2 (7.7) | 2 (6.7) | 1.000 | |
> Quintuple | S436G437E540G581–I51R59N108I164 | 0 (0.0) | 1 (3.8) | 1 (3.3) | 1.000 | |
A436G437K540A581–I51R59N108L164 | 0 (0.0) | 1 (3.8) | 1 (3.3) | 1.000 | ||
A436G437E540A581–N51R59N108I164 | 0 (0.0) | 1 (3.8) | 1 (3.3) | 1.000 | ||
A436G437E540A581–N51R59N108L164 | 0 (0.0) | 2 (7.7) | 2 (6.7) | 1.000 | ||
A436G437E540A581–I51R59N108I164 | 1 (25.0) | 2 (7.7) | 3 (10.0) | 0.360 | ||
A436G437E540A581–I51R59N108L164 | 3 (75.0) | 14 (53.8) | 17 (56.7) | 0.613 | ||