Table 1 The sequence of oligonucleotide primers used in this study

Primary PCR

 MSP 2-1

5′-ATG AAG GTA ATT AAA ACA TTG TCT ATT ATA-3'

 MSP 2-4

5′-TTA TAT GAA TAT GGC AAA AGA TAA AAC AAG-3'

Nested PCR

 MSP 2-2

5′-ACA TTC ATA AAC AAT GCT TAT AAT ATG AGT-3'

 MSP 2-3

5′- GAT TAT TTC TAG AAC CAT GCA TAT GTC CAT -3'

 FC27-1

5′-GCA AAT GAA GGT TCT AAT ACT AAT AG-3'

 FC27-2

5′-GCT TTG GGT CCT TCT TCA GTT GAT TC-3'

 3D7-1

5′-GCA GAA AGT AAG CCT TCT ACT GGT GCT-3'

 3D7-2

5′-GAT TTG TTT CGG CAT TAT TAT GA-3'