Fig. 4
Expression, purification and conformational analysis of the recombinant PfRH5 proteins. a Crude cell lysate was stained with Coomassie blue. b Representative image of SDS–PAGE in which fractions from Ni–NTA column eluted with 500 mM imidazole was shown. c Western blotting of whole cell lysate expressing 6X-His-tag protein. d Synchronous fluorescene of rPfRH53D7 and rPfRH5M at Δλ70. e FTIR spectra of rPfRH53D7 and rPfRH5M. Analysis of secondary structure of f rPfRH53D7 and g rPfRH5M by Far-UV CD spectra (estimated proportions of secondary structural constituents were illustrated in the pie-charts)