Fig. 5
Characterization of binding affinity of PfRH53D7 and PfRH5M with BSG. a FTIR spectra of complexes formed between rPfRH53D7 (blue line) or rPfRH5M (red line) with BSG. CD spectroscopy of b rPfRH53D7–BSG and c rPfRH5M–BSG complexes. Estimated proportion of secondary structural constituents was illustrated in adjoining pie-charts. d Synchronous fluorescence of rPfRH53D7–BSG and rPfRH5M –BSG complexes at Δλ70. e Graphical representation of fluorescence anisotropy estimates of receptor (BSG), ligands (RH53D7 and RH5M), and their complexes (BSG-RH53D7 and BSG-RH5M). Data from ITC showing exothermic interaction of BSG with f rPfRH53D7 and g rPfRH5M at pH 7.0. Each peak indicated an injection of BSG onto respective PfRH5-ligand kept in the ITC cell. The area under the peak is proportional to the amount of heat released during the interaction. ITC profile for binding reactions were generated using 0.1 M phosphate buffer at a temp of 25 °C