Table 1 Oligonucleotide primers and PCR conditions used in this study
From: Kelch 13-propeller polymorphisms in Plasmodium falciparum from Jazan region, southwest Saudi Arabia
Primer name | Sequence (5′-3′) | Size (bp) | PCR condition |
|---|---|---|---|
rPLU5 rPLU6 | cctgttgttgccttaaacttc ttaaaattgttgcagttaaaacg | 1100 | 95 °C × 3 min, 35 cycles (94 °C × 60 s, 60 °C × 90 s, 72 °C × 90 s), 72 °C × 10 min |
rFAL1 rFAL2 | ttaaactggtttgggaaaaccaaatatatt acacaatgaactcaatcatgactacccgtc | 205 | 95 °C × 3 min, 35 cycles (94 °C × 60 s, 55 °C × 90 s, 72 °C × 90 s), 72 °C × 10 min |
rVIV2 rVIV1 | cgcttctagcttaatccacataactgatac acttccaagccgaagcaaagaaagtcctt | 120 | 95 °C × 3 min, 35 cycles (94 °C × 60 s, 55 °C × 90 s, 72 °C × 90 s), 72 °C × 10 min |
K13 PCR F K13 PCR R | gggaatctggtggtaacagc cggagtgaccaaatctggga | 2097 | 95 °C × 10 min, 35 cycles (94 °C × 60 s, 55 °C × 90 s, 72 °C × 90 s), 72 °C × 10 min |
K13 Nested F K13 Nested R | gccttgttgaaagaagcaga gccaagctgccattcatttg | 849 | 95 °C × 5 min, 35 cycles (94 °C × 60 s, 60 °C × 90 s, 72 °C × 90 s), 72 °C × 10 min |