Fig. 4From: Definition of constitutive and stage-enriched promoters in the rodent malaria parasite, Plasmodium yoeliiIndirect immunofluorescence assay of P. yoelii parasites using mosquito- or liver-stage promoters to express GFPmut2. Oocysts were visualized by live fluorescence and DIC microscopy. Oocyst sporozoite, salivary gland sporozoite, and 24-h and 48-h liver stage parasites were stained with anti-GFP antibodies and stage-defining, counterstaining antibodies (CSP, ACP). Previously defined stage-specific expression is indicated by light blue shading behind the respective micrographs. Scale bars are 10 or 20 µmBack to article page