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Fig. 4 | Malaria Journal

Fig. 4

From: Definition of constitutive and stage-enriched promoters in the rodent malaria parasite, Plasmodium yoelii

Fig. 4

Indirect immunofluorescence assay of P. yoelii parasites using mosquito- or liver-stage promoters to express GFPmut2. Oocysts were visualized by live fluorescence and DIC microscopy. Oocyst sporozoite, salivary gland sporozoite, and 24-h and 48-h liver stage parasites were stained with anti-GFP antibodies and stage-defining, counterstaining antibodies (CSP, ACP). Previously defined stage-specific expression is indicated by light blue shading behind the respective micrographs. Scale bars are 10 or 20 µm

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