Fig. 1

RNAi of Aedes aegypti SGS1 reduces the number of sporozoites invading the salivary glands. a Timeline of the knockdown experiment. b aaSGS1 transcript detection from knockdown mosquitoes. Total RNA was extracted from pools of 5 females at 2, 3, 4, 5, and 6 days post injection (dpi) of dsSGS1 or dsEGFP mosquitoes and used in an RT-PCR reaction with specific primers for SGS1 or actin. c SGS1 normalized mRNA abundance by RT-qPCR from mosquitoes silenced by dsSGS1 or dsGFP at 24hs post injection; bars represent standard deviation from two replicates. d Salivary gland sporozoite intensity in dsRNA-treated, P. gallinaceum-infected A. aegypti. Each point represents one pair of salivary glands and the horizontal bars represents the median. A Mann–Whitney U test was used to evaluate statistical significance of parasite intensity of infection. A P value of  < 0.05 was considered statistically significant. e Mean haemolymph sporozoite intensity in dsRNA-treated, P. gallinaceum-infected A. aegypti. n represents the total number of females used to obtain haemolymph. f SGS1b normalized mRNA abundance by RT-qPCR from mosquitoes silenced by dsSGS1 or dsGFP; bars represent standard deviation from two technical replicates