KASP: a genotyping method to rapid identification of resistance in Plasmodium falciparum

Malaria Journal

Table 1 Primers name, gene target, sequence and method for the P. falciparum resistance genotyping

Target	Primer name/reference	Method	Seq. 5′→3′
MDR	MDR1-pfmdr1-1FK76T²¹	RT-PCR MDR A and sequencing	GTTGAACAAAAAGAGTACCGCTG
	MDR2-pfmdr1-1RK76T²¹	RT-PCR MDR A and sequencing	TCGTACCAATTCCTGAACTCAC
	MDR3-pfmdr1-2FK76T²¹	Sequencing	TTTCCGTTTAAATGTTTACCTGC
	MDR4-pfmdr1-2RK76T²¹	Sequencing	CCATCTTGATAAAAAACACTTCTT
	MDR1-1246-pfmdr1-1FD1246Y²¹	RT-PCR MDR B and sequencing	ATGACAAATTTTCAAGATTA
	MDR2-1246-pfmdr1-1FD1246Y²¹	RT-PCR MDR B and sequencing	ACTAACACGTTTAACATCTT
	MDR3-1246-pfmdr1-1FD1246Y²¹	Sequencing	AATGTAAATGAATTTTCAAACC
	MDR4-1246-pfmdr1-1FD1246Y²¹	Sequencing	CATCTTCTCTTCCAAATTTGATA
Cyt B	CytB1-1F²²	RT-PCR/sequencing	CTCTATTAATTTAGTTAAAGCACAC
	CytB2-1R²²	RT-PCR/sequencing	ACAGAATAATCTCTAGCACC
	CytB3- 2F²²	Sequencing	AGCAGTAATTTGGATATGTGGAGG
	CytB4-2R²²	Sequencing	ATTTTTAATGCTGTATCATACCCT
K13	Pfal-k13-2-2PCR²⁰	RT-PCR/sequencing	GCCAAGCTGCCATTCATTTG
K13	Pfal-k13-3-2PCR²⁰	RT-PCR/sequencing	GCCTTGTTGAAAGAAGCAGA

ISSN: 1475-2875