Table 3 Thermal cycle conditions for KASP genotyping reactions
From: KASP: a genotyping method to rapid identification of resistance in Plasmodium falciparum
Step | Description | Temperature | Time | Cycles |
|---|---|---|---|---|
1 | Activation | 94 °C | 15 min | 1 cycle |
2 | Denaturation | 94 °C | 20 s | 10 cycles |
Annealing/elongation | 61–55 °C (drop 0.6 °C per cycle) | 60 s | ||
3 | Denaturation | 94 °C | 20 s | 35 cycles |
Annealing/elongation | 55 °C | 60 s | ||
4 | Read stage (FAM™, HEX™, ROX™) | 30 °C | 60 s |