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Table 1 Details of the Plasmodium spp. proteins expressed in this study

From: A panel of recombinant proteins from human-infective Plasmodium species for serological surveillance

Sp. Official nomenclature Synonym/s Accession number Region expressed Length (aa) Exp. level PlasmoDB, previous ID Addgene ID
P. falciparum MSP1* MSA1, Pf190, Pf195 PF3D7_0930300 V20-S1701 1682 Low Merozoite surface protein 1 47709
AMA1* Pf83, RMA1, PfAMA1 PF3D7_1133400 Q25-T541 517 High Apical membrane antigen 1, PF11_0344 47741
P92* Pf92 PF3D7_1364100 A26-S770 745 Low 6-cysteine protein, PF13_0338 47728
MSP4   PF3D7_0207000 Y29-S253 225 Low Merozoite surface protein 4, PFB0310C 47717
MSP5   PF3D7_0206900.1 N22-S251 230 Low Merozoite surface protein 5, PFB0305C 47718
P12* Pf12, Pfs12 PF3D7_0612700 H26-S323 298 High 6-cysteine protein, MAL6P1, PFF0615C 47725
GAMA PSOP9 PF3D7_0828800 L22-P710 689 Med GPI-anchored micronemal antigen 47747
MSP10*   PF3D7_0620400 H27-S503 477 Med Merozoite surface protein 10, MAL6P1.221, PFF0995C 47719
P38 Pf38, Pfs38 PF3D7_0508000 Q22-S328 307 High 6-cysteine protein, PFE0395C 47727
MSP3.1* MSP3, SPAM PF3D7_1035400 K26-H354 328 Med Merozoite surface protein 3, PF10_0345 47731
P41 Pf41, Pfs41 PF3D7_0404900 K21-S378 358 High 6-cysteine protein, PFD0240C 47739
MSP7   PF3D7_1335100 T28-M351 324 Med Merozoite surface protein 7, PF13_0197 47735
HPzz* PFA0210c PF3D7_0104200 Y24-D466 443 Low StAR-related lipid transfer protein, MAL1P1.33 50821
P. vivax MSP4   PVX_003775 A26-S227 202 Low Merozoite surface protein 4, putative, PV003775 68510
MSP5*   PVX_003770 R22-S367 346 Med Merozoite surface protein 5, PV003770 68511
P12*   PVX_113775 F24-A339 316 Med 6-cysteine protein, PV113775 68516
GAMA   PVX_088910 L21-S749 729 Med GPI-anchored micronemal antigen, putative, PV088910 68522
CyRPA   PVX_090240 T23-D366 344 Med Cysteine-rich protective antigen, putative, PV090240 68525
HPzz*   PVX_081550 R23-F495 473 Low StAR-related lipid transfer protein, putative, PV081550 68532
P38*   PVX_097960 K29-G334 306 Med 6-cysteine protein, PV097960 68518
MSP7.1*   PVX_082700 E22-Y420 399 Med Merozoite surface protein 7 (MSP7), PV082700 68512
MSP3γ MSP3.1 PVX_097670 N21-K845 825 Med Merozoite surface protein 3, PV097670 68506
P41*   PVX_000995 E22-E384 363 Med 6-cysteine protein, PV000995 68519
P. malariae CyRPA   PmUG01_05040800 E20-D358 339 Low Cysteine-rich protective antigen, putative 126817
GAMA   PmUG01_05017200 L22-S764 743 High GPI-anchored micronemal antigen 126818
MSP10*   PmUG01_11043200 N27-S389 363 Low Merozoite surface protein 10, putative 126819
MSP4   PmUG01_04025600 Y29-S206 178 Low Merozoite surface protein 4, putative 126820
MSP5*   PmUG01_04025700 K22-L291 270 High Merozoite surface protein 5 126821
P12 P12p PmUG01_11050400 Y19-S333 315 Low 6-cysteine protein 126822
P38   PmUG01_06016900 M1-A310 310 Low 6-cysteine protein, putative 126823
P41*   PmUG01_03015000 Q23-D374 352 Med 6-cysteine protein, putative 126824
MSP3.10   PmUG01_06022000 K24-I525 522 High Merozoite surface protein 3, putative 126825
MSP3.5   PmUG01_06022600 N21-S562 542 High Merozoite surface protein 3, putative 126826
MSP7.1   PmUG01_12029500 R25-I442 418 High MSP7-like protein, putative 126827
MSP7.4   PmUG01_12029900 K22-V394 373 High MSP7-like protein, putative 126828
P. ovale CyRPA   POVCU1_054880 S20-D359 340 Med Cysteine-rich protective antigen, putative, SBS99762.1 126829
GAMA   PocGH01_05012100 L22-S755 734 High GPI-anchored micronemal antigen, putative 126830
MSP10*   PocGH01_11036900 Y27-S430 404 High Merozoite surface protein 10, putative 126831
MSP4   PocGH01_04023000 N26-S209 184 High Merozoite surface protein 4, putative 126832
MSP5   PocGH01_04023100 F20-S311 292 High Merozoite surface protein 5, putative 126833
P12 P12p PocGH01_11044100 E23-S301 279 Low 6-cysteine protein 126834
P38   PocGH01_10033500 K25-S332 308 Low 6-cysteine protein, putative 126835
P41*   PocGH01_03012400 E16-E375 360 Med 6-cysteine protein, putative 126836
MSP3.5*   PocGH01_10038700 K24-P480 457 Med Merozoite surface protein 3, putative 126837
MSP3.6   PocGH01_10038800 K24-S732 709 Low Merozoite surface protein 3, putative 126838
MSP7.12   PocGH01_12028800 K23-T441 419 Med MSP7-like protein, putative 126839
MSP7.8   PocGH01_12028400 K23-T379 357 Med MSP7-like protein, putative 126840
P. knowlesi CyRPA   PKNH_0515800 N23-E366 344 Med Cysteine-rich protective antigen, putative, PKH_052740 126841
GAMA PSOP9 PKNH_1322900 L21-S700 680 Med GPI-anchored micronemal antigen, putative, PKH_050210 126842
MSP10*   PKNH_1129800 N27-S417 391 High Merozoite surface protein 10, putative, PKH_112880 126940
MSP4   PKNH_0414100 D26-S182 157 Med Merozoite surface protein 4, putative, PKH_041300 126843
MSP5   PKNH_0414200 H22-S354 333 Med Merozoite surface protein 5, PKH_041310 126844
P12*   PKNH_1137300 F24-S323 300 Med 6-cysteine protein, PKH_113620 126845
P38*   PKNH_1025600 K3-S367 365 Low 6-cysteine protein, PKH_102490 126846
P41   PKNH_0303000 E22-E393 372 High 6-cysteine protein, PKH_030970 126847
P92   PKNH_1107200 D24-P857 834 Med 6-cysteine protein, PKH_110660 126848
  1. Protein expression plasmids encoding the named genes from the five Plasmodium species that infect humans are detailed. ‘Region expressed’ refers to the ectodomain regions between the predicted signal sequence peptide and the transmembrane or GPI anchor sequence (if present). Expression levels are given as a guide only given the significant batch-to-batch variability observed using this approach and grouped into ‘high’ (between 5 and 50 μg/mL), ‘medium’ (0.5–5 μg/mL) and ‘low’ (0.005–0.5 μg/mL). Those proteins selected for further serological analysis are marked with an asterisk