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Table 1 Details of the Plasmodium spp. proteins expressed in this study

From: A panel of recombinant proteins from human-infective Plasmodium species for serological surveillance

Sp.Official nomenclatureSynonym/sAccession numberRegion expressedLength (aa)Exp. levelPlasmoDB, previous IDAddgene ID
P. falciparumMSP1*MSA1, Pf190, Pf195PF3D7_0930300V20-S17011682LowMerozoite surface protein 147709
AMA1*Pf83, RMA1, PfAMA1PF3D7_1133400Q25-T541517HighApical membrane antigen 1, PF11_034447741
P92*Pf92PF3D7_1364100A26-S770745Low6-cysteine protein, PF13_033847728
MSP4 PF3D7_0207000Y29-S253225LowMerozoite surface protein 4, PFB0310C47717
MSP5 PF3D7_0206900.1N22-S251230LowMerozoite surface protein 5, PFB0305C47718
P12*Pf12, Pfs12PF3D7_0612700H26-S323298High6-cysteine protein, MAL6P1, PFF0615C47725
GAMAPSOP9PF3D7_0828800L22-P710689MedGPI-anchored micronemal antigen47747
MSP10* PF3D7_0620400H27-S503477MedMerozoite surface protein 10, MAL6P1.221, PFF0995C47719
P38Pf38, Pfs38PF3D7_0508000Q22-S328307High6-cysteine protein, PFE0395C47727
MSP3.1*MSP3, SPAMPF3D7_1035400K26-H354328MedMerozoite surface protein 3, PF10_034547731
P41Pf41, Pfs41PF3D7_0404900K21-S378358High6-cysteine protein, PFD0240C47739
MSP7 PF3D7_1335100T28-M351324MedMerozoite surface protein 7, PF13_019747735
HPzz*PFA0210cPF3D7_0104200Y24-D466443LowStAR-related lipid transfer protein, MAL1P1.3350821
P. vivaxMSP4 PVX_003775A26-S227202LowMerozoite surface protein 4, putative, PV00377568510
MSP5* PVX_003770R22-S367346MedMerozoite surface protein 5, PV00377068511
P12* PVX_113775F24-A339316Med6-cysteine protein, PV11377568516
GAMA PVX_088910L21-S749729MedGPI-anchored micronemal antigen, putative, PV08891068522
CyRPA PVX_090240T23-D366344MedCysteine-rich protective antigen, putative, PV09024068525
HPzz* PVX_081550R23-F495473LowStAR-related lipid transfer protein, putative, PV08155068532
P38* PVX_097960K29-G334306Med6-cysteine protein, PV09796068518
MSP7.1* PVX_082700E22-Y420399MedMerozoite surface protein 7 (MSP7), PV08270068512
MSP3γMSP3.1PVX_097670N21-K845825MedMerozoite surface protein 3, PV09767068506
P41* PVX_000995E22-E384363Med6-cysteine protein, PV00099568519
P. malariaeCyRPA PmUG01_05040800E20-D358339LowCysteine-rich protective antigen, putative126817
GAMA PmUG01_05017200L22-S764743HighGPI-anchored micronemal antigen126818
MSP10* PmUG01_11043200N27-S389363LowMerozoite surface protein 10, putative126819
MSP4 PmUG01_04025600Y29-S206178LowMerozoite surface protein 4, putative126820
MSP5* PmUG01_04025700K22-L291270HighMerozoite surface protein 5126821
P12P12pPmUG01_11050400Y19-S333315Low6-cysteine protein126822
P38 PmUG01_06016900M1-A310310Low6-cysteine protein, putative126823
P41* PmUG01_03015000Q23-D374352Med6-cysteine protein, putative126824
MSP3.10 PmUG01_06022000K24-I525522HighMerozoite surface protein 3, putative126825
MSP3.5 PmUG01_06022600N21-S562542HighMerozoite surface protein 3, putative126826
MSP7.1 PmUG01_12029500R25-I442418HighMSP7-like protein, putative126827
MSP7.4 PmUG01_12029900K22-V394373HighMSP7-like protein, putative126828
P. ovaleCyRPA POVCU1_054880S20-D359340MedCysteine-rich protective antigen, putative, SBS99762.1126829
GAMA PocGH01_05012100L22-S755734HighGPI-anchored micronemal antigen, putative126830
MSP10* PocGH01_11036900Y27-S430404HighMerozoite surface protein 10, putative126831
MSP4 PocGH01_04023000N26-S209184HighMerozoite surface protein 4, putative126832
MSP5 PocGH01_04023100F20-S311292HighMerozoite surface protein 5, putative126833
P12P12pPocGH01_11044100E23-S301279Low6-cysteine protein126834
P38 PocGH01_10033500K25-S332308Low6-cysteine protein, putative126835
P41* PocGH01_03012400E16-E375360Med6-cysteine protein, putative126836
MSP3.5* PocGH01_10038700K24-P480457MedMerozoite surface protein 3, putative126837
MSP3.6 PocGH01_10038800K24-S732709LowMerozoite surface protein 3, putative126838
MSP7.12 PocGH01_12028800K23-T441419MedMSP7-like protein, putative126839
MSP7.8 PocGH01_12028400K23-T379357MedMSP7-like protein, putative126840
P. knowlesiCyRPA PKNH_0515800N23-E366344MedCysteine-rich protective antigen, putative, PKH_052740126841
GAMAPSOP9PKNH_1322900L21-S700680MedGPI-anchored micronemal antigen, putative, PKH_050210126842
MSP10* PKNH_1129800N27-S417391HighMerozoite surface protein 10, putative, PKH_112880126940
MSP4 PKNH_0414100D26-S182157MedMerozoite surface protein 4, putative, PKH_041300126843
MSP5 PKNH_0414200H22-S354333MedMerozoite surface protein 5, PKH_041310126844
P12* PKNH_1137300F24-S323300Med6-cysteine protein, PKH_113620126845
P38* PKNH_1025600K3-S367365Low6-cysteine protein, PKH_102490126846
P41 PKNH_0303000E22-E393372High6-cysteine protein, PKH_030970126847
P92 PKNH_1107200D24-P857834Med6-cysteine protein, PKH_110660126848
  1. Protein expression plasmids encoding the named genes from the five Plasmodium species that infect humans are detailed. ‘Region expressed’ refers to the ectodomain regions between the predicted signal sequence peptide and the transmembrane or GPI anchor sequence (if present). Expression levels are given as a guide only given the significant batch-to-batch variability observed using this approach and grouped into ‘high’ (between 5 and 50 μg/mL), ‘medium’ (0.5–5 μg/mL) and ‘low’ (0.005–0.5 μg/mL). Those proteins selected for further serological analysis are marked with an asterisk