Polymorphic markers for identification of parasite population in Plasmodium malariae

Malaria Journal

Table 1 List of candidate genotyping microsatellite markers for Plasmodium malariae UG01

S. no.	Locus^a	Primer	Primer sequence	T_m (°C)	Location
1	Pm05_707	Forward^b	GGTAGAAGGAGCAACGGACA	63.5	Chromosome 5
1	Pm05_707	Reverse	CGCTCGGGTCATCGTTATTA	61.3	Chromosome 5
2	Pm06_506	Forward^b	TTGTGCGTATGCAACCTTTC	57.6	Chromosome 6
2	Pm06_506	Reverse	CAAAAGGGAAGGAGCACAAA	57.0	Chromosome 6
3	Pm07_429	Forward^b	TTCCTTTTCATCCTCTGCAA	59.1	Chromosome 7
3	Pm07_429	Reverse	CGAATGAGAGTAGTGCGGAAA	62.8	Chromosome 7
4	Pm09_801	Forward^b	TGACTTCGGTTAGAATATGTTTGC	60.7	Chromosome 9
4	Pm09_801	Reverse	TCACACTCCTTTCAATTTCTCA	59.4	Chromosome 9
5	Pm12_426	Forward^b	TTCGTGTTCTCGCTTTCCTC	62.0	Chromosome 12
5	Pm12_426	Reverse	GATCACTTCGCACGGGATAG	61.9	Chromosome 12
6	Pm13_110	Forward^b	TCAAGTGGAATAACCGCAAG	60.0	Chromosome 13
6	Pm13_110	Reverse	CAGACGAGGACTTTCATTTCG	60.4	Chromosome 13

^aNovel microsatellite markers for Plasmodium malariae based on fragment analysis of PCR products (N = 37)
^bForward primers were labelled with 6-FAM for fragment analysis. Thermal cyclin profile: initial denaturation at 94 °C for 5 min, 40 cycles of 94 °C for 1 min, 58–63 °C for 1 min and 72 °C for 1 min, followed by a final extension at 72 °C for 5 min

ISSN: 1475-2875