Fig. 1

Set up of a luminescent assay to evaluate the phagocytosis of late GCT by immortalized mouse C57Bl/6 bone marrow-derived macrophages. a After 2 h incubation with GCT, cells were washed with water (water lysis, striped column) or PBS (no lysis, black column); b BMDM were pre-incubated in the presence (+ CytoD, grey column) or absence (-CytoD, white column) of Cytochalasin D (CytoD) 2 µM for 1 h before incubation with GCT. After 2 h incubation with GCT, the water lysis step was performed and luminescence was measured and expressed as Absolute Luminescent Units (ALU). c BMDM were incubated for different time points with GCT. After the water lysis step luminescence was measured and expressed as ALU. Data are the mean ± sd of 3 independent experiments in quadruplicate. Two-Way ANOVA, Tukey’s multiple comparisons test ****P < 0.0001