Fig. 3

Immortalized mouse C57Bl/6 bone marrow-derived macrophages were treated with early or late GCT at the cell:GCT ratio of 1:1.5, for 2 h. a After washing and lysis, luminescence was read using a Biotek Synergy4 microplate reader. Results are expressed as arbitrary luminescence units (ALU). *p < 0.05; ****P < 0.0001 versus control, One-Way ANOVA, Dunnett’s multiple comparison test. b Linear correlation between the number of GCT and the relative luminescence in two different dose-response curves, one for late GCT, one for early GCT. c Magnification of the lower part of b. The table shows the luminescence emitted (ALU) by the medium alone, or by early or late GCT (BMDM treatment), and the numbers of GCT phagocytized by BMDM extrapolated from the dose-response curve shown in c (GCT number). d BMDM were pre-incubated in the presence (+ CytoD, grey column) or absence (−CytoD, white column) of Cytochalasin D (CytoD) 2 µM for 1 h before incubation with GCT. After 2 h incubation with GCT, the water lysis step was performed and luminescence was measured and expressed as Absolute Luminescent Units (ALU). ***p < 0.001; ****P < 0.0001 versus control, Two-Way ANOVA, Tuckey’s multiple comparison test. Data are the mean ± sd of 3 independent experiments in triplicate