Fig. 5
From: Phagocytosis and activation of bone marrow‐derived macrophages by Plasmodium falciparum gametocytes
Immortalized mouse C57Bl/6 bone marrow-derived macrophages were pre-incubated in the presence (+ CytoD, grey column) or absence (-CytoD, white column) of Cytochalasin D (CytoD) 2 µM for 1 h before incubation with early or late GCT at the cell:GCT ratio of 1:2, for 24 h. The production of NO was evaluated by measuring nitrite in cell supernatants by Griess assay. The production of TNF and MIP-2 was evaluated in the supernatants by ELISA. Data are the mean ± sd of 3 independent experiments in triplicate. *p < 0.05; **p < 0.01; ***p < 0.001; ****P < 0.0001 versus control, Two-Way ANOVA, Tukey’s multiple comparison test