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Fig. 2 | Malaria Journal

Fig. 2

From: Multiplex-RT-PCR-ELISA panel for detecting mosquito-borne pathogens: Plasmodium sp. preserved and eluted from dried blood spots on sample cards

Fig. 2

Gel electrophoresis of multiplex-RT-PCR products. The nucleic acid isolated from each of nine vector-borne pathogens (positive controls) was amplified using the multiplex-RT-PCR, and the product of each reaction was subjected to 2% agarose gel electrophoresis. MW molecular weight marker pUC19 DNA/MspI, ThermoFisher Scientific, Waltham, MA USA) and the following pathogens, MAL malaria, 99 bp, DENV Dengue virus, 511 bp, ZIKA Zika virus, 100 bp, RVFV Rift Valley Fever virus, 69 bp, CHIKV Chikungunya virus, 81 bp, WNV West Nile virus, 116 bp, YFV Yellow Fever virus (YFV 83 bp), ONNV O´nyong-nyong virus, 148 bp, SFV Semliki Forest virus, 230 bp, NeCo Iso negative isolation control, NeCo RT negative reverse transcriptase control, NeCo PCR negative primermix control

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