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Table 1 Sample card extraction

From: Multiplex-RT-PCR-ELISA panel for detecting mosquito-borne pathogens: Plasmodium sp. preserved and eluted from dried blood spots on sample cards

  Klüber methoda Klüber-Grüner method Grüner method [17]
Amount Six paper disks (3 mm) Six paper disks (3 mm) Six paper disks (3 mm)
Time 1 h at 750 rpm 1 h at 750 rpm Gently shaking overnight
Temperature 72 °C 72 °C Room temperature
Buffer/process 300 μl of nuclease-free water. Vortex reaction tubes every 15 min 300 µl PBS-buffer containing Tween 20 and 0.9% NaCl. Vortex reaction tubes every 15 min 300 µl of PBS-buffer containing Tween 20 and 0.9% NaCl
  1. Positive controls spotted on Whatman® 903 protein sample cards were extracted by one of the three methods indicated
  2. aJ. Klüber, Master’s Thesis, unpublished
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