Table 1 Sample card extraction
|  | Klüber methoda | Klüber-Grüner method | Grüner method [17] |
|---|---|---|---|
Amount | Six paper disks (3Â mm) | Six paper disks (3Â mm) | Six paper disks (3Â mm) |
Time | 1Â h at 750Â rpm | 1Â h at 750Â rpm | Gently shaking overnight |
Temperature | 72 °C | 72 °C | Room temperature |
Buffer/process | 300 μl of nuclease-free water. Vortex reaction tubes every 15 min | 300 µl PBS-buffer containing Tween 20 and 0.9% NaCl. Vortex reaction tubes every 15 min | 300 µl of PBS-buffer containing Tween 20 and 0.9% NaCl |