Multiplex-RT-PCR-ELISA panel for detecting mosquito-borne pathogens: Plasmodium sp. preserved and eluted from dried blood spots on sample cards

Table 1 Sample card extraction

	Klüber method^a	Klüber-Grüner method	Grüner method [17]
Amount	Six paper disks (3 mm)	Six paper disks (3 mm)	Six paper disks (3 mm)
Time	1 h at 750 rpm	1 h at 750 rpm	Gently shaking overnight
Temperature	72 °C	72 °C	Room temperature
Buffer/process	300 μl of nuclease-free water. Vortex reaction tubes every 15 min	300 µl PBS-buffer containing Tween 20 and 0.9% NaCl. Vortex reaction tubes every 15 min	300 µl of PBS-buffer containing Tween 20 and 0.9% NaCl

Positive controls spotted on Whatman^® 903 protein sample cards were extracted by one of the three methods indicated
^aJ. Klüber, Master’s Thesis, unpublished

ISSN: 1475-2875