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Table 4 Multiplex-RT-PCR-ELISA

From: Multiplex-RT-PCR-ELISA panel for detecting mosquito-borne pathogens: Plasmodium sp. preserved and eluted from dried blood spots on sample cards

Positive control P. fal DENVa ZIKA RVFV CHIKV WNV YFV ONNV SFV
ELISAb (OD405) 2.31 ± 0.01 1.65 ± 0.07 2.12 ± 0.05 1.72 ± 0.09 1.27 ± 0.04 1.67 ± 0.11 2.04 ± 0.08 1.77 ± 0.07 1.92 ± 0.20
  1. aDENV-2 serotype
  2. bData, normalized based upon the OD405 values generated from pooled influenza A amplicons, are the means ± SEM obtained from triplicate plates. OD readings ≥ 4-times the negative control (0.1 OD405) were judged to be positive [27]
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