Skip to main content

Table 4 Multiplex-RT-PCR-ELISA

From: Multiplex-RT-PCR-ELISA panel for detecting mosquito-borne pathogens: Plasmodium sp. preserved and eluted from dried blood spots on sample cards

Positive control

P. fal

DENVa

ZIKA

RVFV

CHIKV

WNV

YFV

ONNV

SFV

ELISAb (OD405)

2.31 ± 0.01

1.65 ± 0.07

2.12 ± 0.05

1.72 ± 0.09

1.27 ± 0.04

1.67 ± 0.11

2.04 ± 0.08

1.77 ± 0.07

1.92 ± 0.20

  1. aDENV-2 serotype
  2. bData, normalized based upon the OD405 values generated from pooled influenza A amplicons, are the means ± SEM obtained from triplicate plates. OD readings ≥ 4-times the negative control (0.1 OD405) were judged to be positive [27]