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Table 6 Efficacy of multiplex-RT-PCR-ELISA conducted on positive controls eluted from sample cards

From: Multiplex-RT-PCR-ELISA panel for detecting mosquito-borne pathogens: Plasmodium sp. preserved and eluted from dried blood spots on sample cards

Pathogensa P. fal DENV ZIKA RVF CHIKV WNV YFV
Spotted
 ELISA (OD405)
0.77 0.59 0.42 1.49 0.59 0.38 1.32
Not spotted
ELISA (OD405)
2.31 1.65 2.12 1.72 1.27 1.67 2.04
  1. aThe positive controls listed were spotted onto sample cards, eluted by the method of Klüber (unpublished Master’s Thesis) and quantified by multiplex-RT-PCR-ELISA. Multiplex-RT-PCR-ELISA conducted on the same controls not spotted onto cards serves as positive controls. All results were normalized in terms of pooled influenza A amplicons (20 µl/well) added to the microtiter plates
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