Multiplex-RT-PCR-ELISA panel for detecting mosquito-borne pathogens: Plasmodium sp. preserved and eluted from dried blood spots on sample cards

Table 6 Efficacy of multiplex-RT-PCR-ELISA conducted on positive controls eluted from sample cards

Pathogens^a	P. fal	DENV	ZIKA	RVF	CHIKV	WNV	YFV
Spotted ELISA (OD₄₀₅)	0.77	0.59	0.42	1.49	0.59	0.38	1.32
Not spotted ELISA (OD₄₀₅)	2.31	1.65	2.12	1.72	1.27	1.67	2.04

^aThe positive controls listed were spotted onto sample cards, eluted by the method of Klüber (unpublished Master’s Thesis) and quantified by multiplex-RT-PCR-ELISA. Multiplex-RT-PCR-ELISA conducted on the same controls not spotted onto cards serves as positive controls. All results were normalized in terms of pooled influenza A amplicons (20 µl/well) added to the microtiter plates

ISSN: 1475-2875