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Table 8 Concordance rates of Plasmodium sp. spotted on cards

From: Multiplex-RT-PCR-ELISA panel for detecting mosquito-borne pathogens: Plasmodium sp. preserved and eluted from dried blood spots on sample cards

Sample card Concordanta Discordanta % Concordanceb Cohen’s κc
   +/+  −/− ±  −/ +    
Sera, n = 132 16/27 102/105 3/105 11/27 89.39% 0.634
whole blood, n = 132 27/27 103/105 2/105 0/27 98.48% 0.955
  1. Sera and whole blood samples obtained from 132 children suspected of having malaria were spotted onto cards. Subsequently, the spots were eluted by the method of Klüber (unpublished Master’s Thesis) and quantified by multiplex-RT-PCR-ELISA
  2. aKey: +/+, true positive; −/−, true negative; ± , false positive; −/+ , false negative
  3. bCompared to the results of multiplex-RT-PCR-ELISA performed directly on the same, non-spotted patient sample
  4. cInterrater reliability where κ 0.61–0.80 = substantial agreement and κ 0.81–0.99 = near perfect agreement
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