Fig. 5

Time-of-day variation in P. berghei transmission traits. a Female gametocyte densities, b male gametocyte densities, c exflagellation events per male gametocyte and d number of ookinetes per female gametocyte. Shown are fitted 24 h Circwave rhythms (solid lines) with mean ± SEM (dashed line and shading) and individual infection data (points). Fits that are non-significant (P > 0.05) are labelled NS and provided for illustration of the trend. Black and white bars represent lights ON (white) and lights OFF (black) and time is given in Zeitgeber time (ZT) in which ZT0 = lights on. Infections (n = 5) were sampled every 4 h on days 5-6PI from wild type MF1 mice pre-treated with phenylhydrazine