Genetic diversity and natural selection on the thrombospondin-related adhesive protein (TRAP) gene of Plasmodium falciparum on Bioko Island, Equatorial Guinea and global comparative analysis

Table 4 Information of mutations at T cell epitopes and mutation effect prediction

Region	Mutations	Frequency in global	Frequency in Bioko	Distribution (Africa%/Asia%)	Polyphen score	ΔΔG
A-Domain	E 46Q	24%	32%	75/25	0.893*	− 0.211536
	E 46R	0%(7/1406)	0%	100/0	0.732*	0.0221299
	L 49 V	1%	3%	100/0	0.944*	1.12894
	I116S	13%	19%	99/1	0.924*	0.342025
	I116T	0%(2/1406)	0%	100/0	0.965**	− 0.386652
	K119R	13%	16%	52/48	0.020	− 0.194173
	L122I	2%	7%	100/0	0.965**	0.571158
	S123N	19%	7%	57/43	0.000	− 0.26044
	S123R	0%(2/1406)	0%	50/50	0.184	− 0.349001
	Y128F	21%	28%	98/2	0.965**	0.770957
	K130R	93%	94%	49/51	0	0.0703724
	T134S	37%	24%	43/57	0.463*	0.705496
TSR	A219E	2%	0%	8/92	0.122	− 0.166547
	A219T	2%	8%	87/13	0.954*	− 0.345222
	E221A	0%(6/1406)	1%	100/0	0.924*	0.128533
	G228V	3%	3%	100/0	0.997**	0.411189
DIV	R285G	1%	0%	100/0	0.663*	1.3286
	R285S	1%	0%	100/0	0.663*	1.16481
	L287P	97%	95%	51/49	0.000	0.546262
	R290W	29%	61%	72/28	0.001	0.336084
	D297H	54%	29%	47/53	0.000	− 0.336216
	D297E	5%	8%	92/8	0.000	− 0.352555
	D297Q	9%	14%	25/75	0.000	− 0.524996
	E298D	2%	0%	100/0	0.544*	− 0.0333006
	E298K	0%(4/1406)	0%	100/0	0.893*	− 1.10525
	P299S	1%	5%	100/0	0.958**	1.17995
	S419P	96%	90%	51/49	0.000	− 0.8004
	K421N	29%	41%	90/10	0.051	1.41483
	V422I	0%(3/1406)	2%	100/0	0.455*	− 0.147794
TM (DV)	Y526F	4%	0%	0/100	0.051	0.56536

Polyphen score with * indicated possibly damaging mutation; Polyphen score with ** indicated probably damaging mutation. Polyphen score without * indicated benign mutation

ISSN: 1475-2875