Table 1 Sequences of primers designed for P. vivax and P. falciparum
From: Multicopy targets for Plasmodium vivax and Plasmodium falciparum detection by colorimetric LAMP
Specie | LAMP | Target | Primers Sequence (5ʹ→3 ʹ ) | |
|---|---|---|---|---|
P. vivax | PvCOX1-LAMP | COX1 | F3 | GAATAATTGCACAAGAAAATGTTAAC |
B3 | GCAACAGGAGATAAAGACATAAGTGA | |||
FIP | CAAGTTCTGGAGAACCACATAAAATTG– CCAGGATTATTTGGAGGATTCG | |||
BIP | GTCATTTTATCTACAGCAGCAGAATTT- TGGTGGATATAAAGTCCATCCAGT | |||
Pvr47-LAMP | Pvr47 | F3 | AACACCTCCCACCAATCA | |
B3 | GTGAATTATCGAAGGCATAA | |||
FIP | TACGCGGAAAATCAGAACAATTCAT- GTGCCAATTTTTTTTTGCGG | |||
BIP | ATCTTTCGCTTATCCATTCATCGA-TAGTGACAAAACATAAACACAGC | |||
P. falciparum | PfEMP1-LAMP | EMP1 | F3 | CCGACAAAACTTTCACCCAA |
B3 | CTGTTGTGTTGTTACCACTAGG | |||
FIP | GGTGTAACCACTATCAGTTCCACTA- AGTGGTAAATACAGAGGCAAAC | |||
BIP | TCCGAAAGTGAGTATGAAGAATTGG- ATGTTTTATATTTAGGACTACCTGG | |||
Pfr364-LAMP | Pfr364 | F3 | CACTAGGTACGCCAACAT | |
B3 | ACCCACAATTTTGATTGAGATG | |||
FIP | GTAGACACCATATGGTACCACGTA- GGATGTGTCTATCATATAGTCCG | |||
BIP | TTGTACCCAATTTTCCCCTAGCA- GTGTGTGCAACATCATAATCATCA |