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Table 4 Sensitivity and specificity of Pfr364 and EMP1 targets to detect Plasmodium falciparum

From: Multicopy targets for Plasmodium vivax and Plasmodium falciparum detection by colorimetric LAMP

  Mini spin column Boil & Spin
Pfr364-LAMP PfEMP1-LAMP Pfr364-LAMP PfEMP1-LAMP
P. falciparum infection detected by microscopy & PCR 100.0% 95 CI 56.1–100% 100.0% 95 CI 56.1–100% 100.0% 95 CI 56.1–100% 100.0% 95 CI 56.1–100%
n = 7 n = 7 n = 7 n = 7 n = 7
P. falciparum submicroscopic infection detected only by PCR 50.0% 95 CI 29.0–71% 72.2% 95 CI 46.4–89.3% 33.3% 95 CI 14.4–58.9% 22.2% 95 CI 7.4–48.1%
n = 18 n = 9 n = 13 n = 6 n = 4
Non P. falciparum infection detected by microscopy & PCR 99.7% 95 CI 97.8–100% 99.0% 95 CI 96.8–99.7% 99.7% 95 CI 97.8–100% 99.7% 95 CI 97.8–100%
n = 287 n = 286 n = 284 n = 286 n = 286
Sensitivitya 64.0% 95 CI 45.2–82.8% 80.0% 95 CI 64.3–95.7% 52.0% 95 CI 32.4–71.6% 44.0% 95 CI 24.5–63.5%
Specificitya 99.7% 95CI 99–100% 99.0% 95 CI 97.8–100% 99.7% 95CI99–100% 99.7% 95CI 99–100%
Agreement Cohen’s Kappaa 0.745 0.595–0.896 0.820 0.698–0.941 0.646 0.470–0.823 0.572 0.381–0.764
  1. aReference method: real-time PCR described by Mangold et al. [40]