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Fig. 3 | Malaria Journal

Fig. 3

From: Activity of Plasmodium vivax promoter elements in Plasmodium knowlesi, and a centromere-containing plasmid that expresses NanoLuc throughout the parasite life cycle

Fig. 3

Selection of drug-resistant P. knowlesi harbouring markers under the control of P. vivax regulatory sequences. Drug selection with 1 nM WR99210 was initiated 72 h after addition of plasmid-loaded RBCs to parasite cultures. A Parasitaemia counts by microscopy of cultures transfected with plasmids pvhsp70D-pfcam-Luc and pkef1D-pfcam-Luc over 16 days of drug selection. B Luminescence measurements from samples of the transformant cultures presented in A. C Parasitaemia counts by microscopy of cultures transfected with plasmid pvcen-pvhsp70-D-NanoLuc in three independent experiments. D Luminescence measurements from samples of the transformant cultures presented in C. LUs, luminescence units; mock, control transformation experiments performed in parallel without plasmid; parasitaemia, percentage of RBCs infected with P. knowlesi parasites (counts of 1000 RBCs)

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Malaria Journal

ISSN: 1475-2875

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