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Table 3 Model-predicted metabolic enzymes that vary substantially over time

From: Inter-study and time-dependent variability of metabolite abundance in cultured red blood cells

Metabolic pathway Metabolic reaction name Absolute flux span (mmol h−1 gDW−1)
Pure 1 -Hxn  + Mev  + Fos Pure 2
Bicarbonate buffer system Bicarbonate transport (\({\text{C}}{\text{l}}^{-}\text{/HC}{\text{O}}_{3}^{-}\) exchange) 0.45 0.53 0.65 0.27 0.81
Carbonic anhydrase 0.45 0.53 0.65 0.27 0.81
Glutamate metabolism Glutathione oxidoreductase 0.91 1.06 1.30 0.53 1.61
Glycolysis Enolase 1.19 0.53 0.87 0.60 1.57
Glyceraldehyde-3-phosphate dehydrogenase 1.19 0.53 0.87 0.60 1.57
Glucose transport (uniport) 0.46 0.28 0.55 0.27 0.87
l-Lactate reversible transport via proton symport 1.19 0.53 0.87 0.60 1.57
l-Lactate dehydrogenase 1.19 0.53 0.87 0.60 1.57
Phosphoglycerate kinase 1.19 0.53 0.87 0.60 1.57
Phosphoglycerate mutase 1.19 0.53 0.87 0.60 1.57
Pyruvate kinase 1.19 0.53 0.87 0.60 1.57
Hexokinase (d-glucose:ATP) 0.46 0.28 0.55 0.27 0.87
Triose-phosphate isomerase 0.73 0.25 0.35 0.27 0.68
Ion transport K+-Cl cotransport 0.45 0.53 0.65 0.27 0.81
H+ exchange 0.36 0.13 0.71 0.27 1.41
Na+/K+ ATPase 0.92 0.25 0.45 0.25 0.85
Oxidative phosphorylation Inorganic diphosphatase 0.29 0.15 0.32 0.14 0.30
Pentose phosphate pathway Glucose 6-phosphate dehydrogenase 0.46 0.53 0.65 0.27 0.81
Phosphogluconate dehydrogenase 0.46 0.53 0.65 0.27 0.81
6-Phosphogluconolactonase 0.46 0.53 0.65 0.27 0.81
Ribulose 5-phosphate 3-epimerase 0.31 0.35 0.42 0.20 0.56
  1. A metabolic enzyme with a flux value that differs by more than 0.1 mmol per hour per gram dry weight (gDW) of red blood cell (RBC) for at least four time points between two studies is considered a substantially variable metabolic flux. Approximately 95% of the metabolic fluxes had a standard deviation of less than 0.1 mmol per hour per gram dry weight of RBC between two study conditions. The absolute difference between the maximum and minimum metabolic flux value for the simulated duration of the experiment. We have provided the maximum and minimum values of each metabolic enzyme in Additional file 4