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Table 3 Key points of the methodology to detect pfhrp2/3 deletions by molecular analysis

From: Impact of Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions on malaria control worldwide: a systematic review and meta-analysis

First author Method of malaria diagnosis/confirmation N total N cases N pfhrp Genes study by molecular analysis (Pfhrp2/Pfhrp3/pfhrp2/3/Flanking genes) Method to test DNA quality Elimination for parasitaemia ≤ 5 p/µL
Abdallah [19] PCR 68 68 68 pfhrp2, pfhrp3, pfhrp2/3, flanking genes PCR NA
Okoth [39] Microscopy/PCR 203 175 175 pfhrp2, pfhrp3, pfhrp2/3, flanking genes PCR, qPCR No
Akinyi [57] PCR 188 188 188 pfhrp2, flanking genes PCR NA
Amoah [74] RDT, microscopy/PCR 558 288 288 pfhrp2, pfhrp, pfhrp2/3 PCR NA
Atroosh [48] RDT, microscopy 622 189 189 pfhrp2 PCR NA
Baker [15] RDT, microscopy 458 458 458 pfhrp2, pfhrp3 NA NA
Baldeviano [59] Microscopy/PCR 210 54 54 pfhrp2 PCR NA
Berhane [14] RDT, microscopy/PCR 51 50 50 pfhrp2, pfhrp3, pfhrp2/3, flanking genes PCR NA
Berzosa [21] RDT/PCR 1724 1724 122 pfhrp2, pfhrp3, pfhrp2/3 PCR NA
Beshir [49] RDT, microscopy/PCR 274 131 131 pfhrp2, pfhrp3, pfhrp2/3, flanking genes qPCR Yes
Bharti [24] RDT, microscopy/PCR 1521 1521 50 pfhrp2, pfhrp3, pfhrp2/3, flanking genes qPCR No
Deme [38] PCR 74 74 NA NA PCR NA
Dong [58] Microscopy/PCR 306 306 306 pfhrp2 PCR NA
Dorado [20] RDT, microscopy/PCR 374 365 365 pfhrp2, pfhrp3, pfhrp2/3, flanking genes PCR NA
Fontecha [41] Microscopy/PCR 128 128 128 pfhrp2, pfhrp3, pfhrp2/3, flanking genes PCR NA
Funwei [69] RDT, microscopy/PCR 511 340 66 pfhrp2, pfhrp3, pfhrp2/3 PCR NA
Gupta [22] RDT, microscopy/PCR 9124 1162 69 pfhrp2, pfhrp3 PCR, qPCR Yes
Gamboa [18] RDT, microscopy/PCR 157 157 157 pfhrp2, pfhrp3, pfhrp2/3, flanking genes PCR NA
Herman [43] RDT/PCR 9317 2695 7 pfhrp2 PCR NA
Kobayashi [52] RDT, microscopy/PCR 5167 1189 36 pfhrp2 qPCR Yes
Koita [28] RDT, microscopy 723 480 37 pfhrp2 PCR NA
Kozycki [11] RDT, microscopy 8757 3291 370 pfhrp2 PCR Yes
Kreidenweiss [55] RDT/PCR 200 200 95 pfhrp2 PCR, qPCR Yes
Kumar Bharti [71] RDT/PCR 1392 1392 1392 pfhrp2, pfhrp3, pfhrp2/3 PCR NA
Kumar [42] RDT, microscopy/PCR 48 48 48 pfhrp2, pfhrp3, pfhrp2/3, flanking genes PCR NA
Kumar [50] RDT/PCR 140 48 48 pfhrp2, pfhrp3 qPCR No
Laban [54] RDT, microscopy/PCR 3292 61 61 pfhrp2 PCR, qPCR Yes
Li [45] RDT/PCR 97 97 97 pfhrp2, pfhrp2/3 qPCR No
Maltha [13] RDT, Microscopy/PCR 182 74 74 pfhrp2, pfhrp3, pfhrp2/3 qPCR No
Menegon [23] Microscopy/PCR 144 144 144 pfhrp2, pfhrp3, pfhrp2/3, flanking genes PCR NA
Murillo Solano [63] RDT, microscopy/PCR 115 100 100 pfhrp2, pfhrp3, pfhrp2/3, flanking genes NA NA
Mussa [61] RDT/PCR 59 26 26 pfhrp2 PCR NA
Nderu [9] PCR 400 400 400 pfhrp2, pfhrp3 PCR NA
Nderu [60] RDT, microscopy/PCR 80 80 80 pfhrp2 PCR NA
Owusu [47] RDT, microscopy/PCR 401 62 8 pfhrp2, pfhrp3, pfhrp2/3, flanking genes PCR NA
Okoth [44] Microscopy/PCR 4 4 4 pfhrp2, pfhrp3, pfhrp2/3 PCR NA
Parr [46] RDT, microscopy/PCR 7137 2752 2752 pfhrp2, pfhrp3, pfhrp2/3 qPCR No
Pati [25] RDT, Microscopy/PCR 1058 384 384 pfhrp2, pfhrp3, pfhrp2/3, flanking genes PCR NA
Plucinski [51] RDT/PCR 1267 458 5 pfhrp2, pfhrp3, pfhrp2/3 qPCR No
Rachid Viana [40] Two RDT, Microscopy 223 223 223 pfhrp2, pfhrp3, pfhrp2/3, flanking genes PCR NA
Ramutton [37] RDT/PCR 3826 77 77 pfhrp2, pfhrp3 PCR, qPCR Yes
Ranadive [53] RDT/PCR 1353 162 9 pfhrp2 PCR, qPCR Yes
Sáenz [56] Microscopy/PCR 32 32 32 pfhrp2, pfhrp3, pfhrp2/3 PCR NA
Thomson [12] RDT, microscopy/PCR 911 718 411 pfhrp2, pfhrp3, pfhrp2/3 PCR, qPCR Yes
Trouvay [73] RDT, microscopy/PCR 359 221 221 pfhrp2, pfhrp3 PCR NA
Willie [30] RDT/PCR 169 169 137 pfhrp2 PCR NA
Willie [62] RDT, microscopy/PCR 260 73 73 pfhrp2 PCR NA
Wurtz [16] RDT, microscopy/PCR 136 125 125 pfhrp2, pfhrp3 qPCR No
  1. N total total of samples included in the study, N cases total of P. falciparum confirmed cases included in the study, N pfhrp no. of samples included for molecular analysis (PCR), pfhrp2/3 double deletion pfhrp2 + pfhrp, NA not applicable