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Table 1 Detection of parasite containing wells per group transfected group

From: Improved Plasmodium falciparum dilution cloning through efficient quantification of parasite numbers and c-SNARF detection

 

Plate number

Positive wells

Detected modified/integrated loci

Group A

Plate 1

8

0

Plate 2

42

Invalidated

Plate 3

24

2

Group B

Plate 1

26

1

Plate 2

32

2

Plate 3

24

2

Group C

Plate 1

18

12

Plate 2

16

Not tested

Plate 3

21

Not tested

  1. Group A represents three experiments that used manual determination of parasitaemia and total cell numbers with an estimated 0.3 parasites/well in limiting dilution microplates. Group B consists of three experiments using limiting dilution setup using flow cytometry with EtBr-DNA staining and 0.3 parasites/well. Group C consists of three trials of limiting dilution using flow cytometry, the SYBR Green I reagent, and 0.2 parasites/well. The column “Detected Modified/integrated Loci” shows the number of detected modified loci in each plate
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Malaria Journal

ISSN: 1475-2875

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