Fig. 4

Western blot of vacuolar preparations from ccc1Δ S. cerevisiae cells. 7 µg of protein from vacuoles prepared from ccc1Δ yeast cells that either harboured ‘empty’ pESC-Leu (vector) or overexpressed wild type sPfVIT transporter was loaded onto each lane of the gel. The left-hand panel shows the molecular weight markers. Localization of heterologously overexpressed sPfVIT to the yeast vacuole was confirmed by detection of the His-tag of the recombinant protein