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Table 2 Annealing positions of the forward and reverse primers of primer pairs 1 and 2

From: Evaluation of a novel real-time PCR assay for the detection, identification and quantification of Plasmodium species causing malaria in humans

Plasmodium species

GenBank accession number

Annealing positions of the forward and reverse primers of primer pair 1a

Annealing positions of the forward and reverse primers of primer pair 2a

P. falciparum

LR605957.1

Forward: 339–357

Reverse: 434–454

Not applicable

P. vivax

KY923424.1

Forward: 1493–1511

Reverse: 1588–1608

Forward: 1941–1971

Reverse: 2122–2152

P. malariae

AB489194.1

Forward: 1560–1578

Reverse: 1655–1675

Not applicable

P. ovale wallikeri

HQ712053.1

Forward: 1038–1056

Reverse: 1133–1153

Not applicable

P. ovale curtisi

HQ712052.1

Forward: 1038–1056

Reverse: 1133–1153

Not applicable

P. knowlesi

LR701176.1

Forward: 340–358

Reverse: 435–455

Forward: 781–811

Reverse: 962–992

P. cynomolgi

AB444125.1

Forward: 1605–1623

Reverse: 1700–1720

Forward: 2051–2081

Reverse: 2232–2262

  1. See main text for the primer sequences. The expected amplicon sizes of primer pairs 1 and 2 are 116 and 212 base pairs, respectively
  2. aAccording to the deposited sequences in GenBank under the shown accession numbers