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Table 5 Within-run and between-run precisions of P. falciparum parasitaemia determined using the MC004 assay

From: Evaluation of a novel real-time PCR assay for the detection, identification and quantification of Plasmodium species causing malaria in humans

Level Average P. falciparum parasitaemia per level (%) Within-run precision
SD/CV
Between-run precision
SD/CV
1 27.27 1.257/4.6 2.133/7.8
2 2.15 0.363/16.9 0.363/16.9
3 0.16 0.022/13.6 0.022/13.6
4 0.09 0.012/14.4 0.012/14.4
  1. Five replicates per run were tested over five runs at four levels of parasitaemia. Parasitaemia was calculated from the Cq-value using the equation of the calibration curve (Fig. 3)
  2. SD standard deviation, CV coefficient of variation