Skip to main content

Table 5 Within-run and between-run precisions of P. falciparum parasitaemia determined using the MC004 assay

From: Evaluation of a novel real-time PCR assay for the detection, identification and quantification of Plasmodium species causing malaria in humans

Level

Average P. falciparum parasitaemia per level (%)

Within-run precision

SD/CV

Between-run precision

SD/CV

1

27.27

1.257/4.6

2.133/7.8

2

2.15

0.363/16.9

0.363/16.9

3

0.16

0.022/13.6

0.022/13.6

4

0.09

0.012/14.4

0.012/14.4

  1. Five replicates per run were tested over five runs at four levels of parasitaemia. Parasitaemia was calculated from the Cq-value using the equation of the calibration curve (Fig. 3)
  2. SD standard deviation, CV coefficient of variation
Back to article page

Malaria Journal

ISSN: 1475-2875

Contact us